NGS and RNA-seq

Topics covered

  • Background of RNA-seq
  • Application of RNA-seq (what RNA-seq can do?)
  • Available sequencing platforms and strategy and which one to choose
  • RNA-seq data analysis
    • Read processing and quality assessment
    • De novo assembly
    • Alignment to reference genome/transcriptome
    • Differentially expressed gene identification
    • Downstream analysis using Plant MetGenMAP

Estimated Time

  • Lecture and examples: 2:00 h

Material

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