Mapping NGS Data

Prerequisites

  • Please, install Virtual Box and the Linux virtual machine we created for this course on your laptop. You will find all the information you need in this link.
  • Download the shell script run_tuxedo.sh and move it to your Virtual Machine Desktop.
  • Install Tablet software on the Virtual Machine for this session.

Topics covered

  • Overview of NGS sequence assembly
  • Reference-guided RNA-seq assembly with Tophat2
  • RNA-expression analysis with Cufflinks
  • Overview of other useful tools for NGS analysis

Estimated Time

  • Lecture and examples: 2:00 h

Materials

 

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